Coding

Part:BBa_K557000

Designed by: Yinghong Lan   Group: iGEM11_USTC-China   (2011-10-02)

CheZ: phosphatase which dephosphorylates CheY-P and thus plays an important role in E.Coli chemotaxi

This part encodes CheZ, which is a phosphatase which dephosphorylates CheY-P and causes the flagellum to rotate counterclockwise. E.coli lacking the cheZ gene cannot dephosphorylate CheY-P, tumble incessantly, and are thus nonmotile while inducing the expression of CheZ can restore motility in a CheZ knockout strain by reactivating wild-type chemotaxis.

EcoliCheZ.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme/phosphorylation
Parameters
chassisE.coli lacking CheZ
targetCheY-P