Coding
Part:BBa_K557000
Designed by: Yinghong Lan Group: iGEM11_USTC-China (2011-10-02)
CheZ: phosphatase which dephosphorylates CheY-P and thus plays an important role in E.Coli chemotaxi
This part encodes CheZ, which is a phosphatase which dephosphorylates CheY-P and causes the flagellum to rotate counterclockwise. E.coli lacking the cheZ gene cannot dephosphorylate CheY-P, tumble incessantly, and are thus nonmotile while inducing the expression of CheZ can restore motility in a CheZ knockout strain by reactivating wild-type chemotaxis.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/enzyme/phosphorylation
chassis | E.coli lacking CheZ |
target | CheY-P |